Portal de investigación
High vancomycin MICs within the susceptible range in Staphylococcus aureus bacteraemia isolates are associated with increased cell wall thickness and reduced intracellular killing by human phagocytes
Autores de CIPF
Participantes ajenos a CIPF
- Falcon, R
- Albert, E
- Madrid, S
- Oltra, R
- Gimenez, E
- Vinuesa, V
- Gozalbo, D
- Gil, ML
- Navarro, D
Abstract
Vancomycin minimum inhibitory concentrations (MICs) at the upper end of the susceptible range for Staphylococcus aureus have been associated with poor clinical outcomes of bloodstream infections. We tested the hypothesis that high vancomycin MICs in S. aureus bacteraemia isolates are associated with increased cell wall thickness and suboptimal bacterial internalisation or lysis by human phagocytes. In total, 95 isolates were evaluated. Original vancomycin MICs were determined by Etest. The susceptibility of S. aureus isolates to killing by phagocytes was assessed in a human whole blood assay. Internalisation of bacterial cells by phagocytes was investigated by flow cytometry. Cell wall thickness was evaluated by transmission electron microscopy. Genotypic analysis of S. aureus isolates was performed using a DNA microarray system. Vancomycin MICs were significantly higher (P=0.006) in isolates that were killed suboptimally (killing index <60%) compared with those killed efficiently (killing index >70%) and tended to correlate inversely (P=0.08) with the killing indices. Isolates in both killing groups were internalised by human neutrophils and monocytes with comparable efficiency. The cell wall was significantly thicker (P=0.03) in isolates in the low killing group. No genotypic differences were found between the isolates in both killing groups. In summary, high vancomycin MICs in S. aureus bacteraemia isolates were associated with increased cell wall thickness and reduced intracellular killing by phagocytes. (C) 2016 Elsevier B.V. and the International Society of Chemotherapy. All rights reserved.
Datos de la publicación
- ISSN/ISSNe:
- 0924-8579, 1872-7913
- Tipo:
- Article
- Páginas:
- 343-350
- PubMed:
- 27056298
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS ELSEVIER SCIENCE BV
Citas Recibidas en Web of Science: 10
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- No hay documentos
Filiaciones
Keywords
- Vancomycin; Minimum inhibitory concentration (MIC); Staphylococcus aureus; Intracellular killing; Cell wall thickness; Human phagocytes
Proyectos asociados
Caracterización molecular de células propagadoras de cáncer de pulmón como herramienta predictiva y para el diseño de terapias personalizadas
Investigador Principal: ROSA M FARRÀS RIVERA
INSTITUTO DE SALUD CARLOS III . 2016