Differentiation of Human Pluripotent Stem Cells (hPSCs) into Brown-Like Adipocytes.

Autores de CIPF

• 

  Stefania Carobbio

  Autor

• 

  Antonio Vidal Puig

  Autor

Grupos de Investigación

• Laboratorio de Obesidad, Diabetes y Cormobilidades

  

  Jefe/a de Grupo Junior

  Stefania Carobbio

• Unidad Mixta CIPF-CAMBRIDGE de Obesidad y Regulación Metabólica

  

  Jefe/a de Grupo

  Antonio Vidal Puig

Abstract

Increasing brown adipose tissue (BAT) mass and activation is a therapeutic strategy to prevent and treat obesity and associated complications. Obese and diabetic patients possess less BAT; thus, finding an efficient way to expand their mass is necessary. There is limited knowledge about how human BAT develops, differentiates, and is optimally activated. Accessing human BAT is challenging, given its scarcity and anatomical dispersion. These constraints make detailed BAT-related developmental and functional mechanistic studies in human subjects virtually impossible. We have developed a new chemically defined protocol for differentiating human pluripotent stem cells (hPSCs) into bona fide brown adipocytes (BAs) that overcomes current limitations. This protocol recapitulates step by step the physiological developmental path of human BAT.

Keywords

• Development, Developmental path, Differentiation, Human brown adipocytes, Human pluripotent stem cells