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Progress In Stem Cells Based Replacement Therapy for Retinal Pigment Epithelium: In Vitro Differentiation to In Vivo Delivery

Fecha de publicación: Fecha Ahead of Print:

Autores de CIPF

Participantes ajenos a CIPF

  • Gupta, S
  • Lytvynchuk, L
  • Ardan, T
  • Studenovska, H
  • Sharma, R
  • Faura, G
  • Eide, L
  • Verma, RS
  • Znaor, L
  • Stieger, K
  • Motlik, J
  • Petrovski, G
  • Bharti, K

Grupos de Investigación

Abstract

Retinal pigment epithelium (RPE) is a critical cell monolayer forming the blood-retina-barrier (BRB) and a permeable bridge between the choriocapillaris and the retina. RPE is also crucial in maintaining photoreceptor function and for completing the visual cycle. Loss of the RPE is associated with the development of degenerative diseases like age-related macular degeneration (AMD). To treat diseases like AMD, pluripotent stem cell-derived RPE (pRPE) has been recently explored extensively as a regenerative module. pRPE like other ectodermal tissues requires specific lineage differentiation and long-term in vitro culturing for maturation. Therefore, understanding the differentiation process of RPE could be useful for stem cell based RPE derivation. Developing pRPE-based transplants and delivering them into the subretinal space is another aspect that has garnered interest in the last decade. In this review, we discuss the basic strategies currently employed for stem cell-based RPE derivation, their delivery, and recent clinical studies related to pRPE transplantation in patients. We have also discussed a few limitations with in vitro RPE culture and potential solutions to overcome such problems which can be helpful in developing functional RPE tissue.

Datos de la publicación

ISSN/ISSNe:
2157-6564, 2157-6580

Stem Cells Translational Medicine  WILEY

Tipo:
Review
Páginas:
536-552
PubMed:
37459045

Citas Recibidas en Web of Science: 13

Documentos

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Métricas

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Keywords

  • retinal pigment epithelium; induced pluripotent stem cells; embryonic stem cells; cell delivery; differentiation

Campos de Estudio

Financiación

Czech Science Foundation (18-04393S)
European Union
IAPG institutional support RVO (67985904)
Norway Grants and Technology Agency of the Czech Republic (TO01000107)

Proyectos asociados

Evaluation of cell therapy using genetically corrected RPE cells in small and large animals for the treatment of hereditary retinal dystrophies

Investigador Principal: SLAVEN ERCEG VUKICEVIC

FUNDACION LA MARATO DE TV3 . 2021

Estrategia preclínica para la modificación del balnace de los astrocitos "buenos" y "malos" para el crecimineto axonal después de lesión de la médula espinal

Investigador Principal: SLAVEN ERCEG VUKICEVIC

INSTITUTO DE SALUD CARLOS III . 2022

Evaluacion preclínica de terapia celular usando las células epiteliales pigmentarias corregidas genéticamente de los pacientes con Retinitis pigmentaria asociada a MERTK

Investigador Principal: SLAVEN ERCEG VUKICEVIC

CONSELLERIA DE EDUCACION . 2022

Testing neurogenic potential of Induced Pluripotent Stem cells derived from Alzeheimer's disease patients. NEURAD.

Investigador Principal: SLAVEN ERCEG VUKICEVIC

SCIENCE FUND OF THE REPUBLIC OF SERBIA . 2020

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