eIF5A is activated by virus infection or dsRNA and facilitates virus replication through modulation of interferon production

Fecha de publicación: 27/07/2022

Autores de CIPF

Rosa M Farràs Rivera

Autor

Participantes ajenos a CIPF

Seoane, R
Llamas-Gonzalez, YY
Vidal, S
El Motiam, A
Bouzaher, YH
Fonseca, D
Garcia-Sastre, A
Gonzalez-Santamaria, J
Rivas, C

Grupos de Investigación

Laboratorio de Señalización Oncogénica

Jefe/a de Grupo

Rosa M Farràs Rivera

Abstract

Active hypusine-modified initiation elongation factor 5A is critical for cell proliferation and differentiation, embryonic development, and innate immune response of macrophages to bacterial infection. Here, we demonstrate that both virus infection and double-stranded RNA viral mimic stimulation induce the hypusination of eIF5A. Furthermore, we show that activation of eIF5A is essential for the replication of several RNA viruses including influenza A virus, vesicular stomatitis virus, chikungunya virus, mayaro virus, una virus, zika virus, and punta toro virus. Finally, our data reveal that inhibition of eIF5A hypusination using the spermidine analog GC7 or siRNA-mediated downmodulation of eIF5A1 induce upregulation of endoplasmic reticulum stress marker proteins and trigger the transcriptional induction of interferon and interferon-stimulated genes, mechanisms that may explain the broad-spectrum antiviral activity of eIF5A inhibition.

Datos de la publicación

ISSN/ISSNe:: 2235-2988, 2235-2988
Tipo:: Article
Páginas:: 960138-960138
DOI:: 10.3389/fcimb.2022.960138
PubMed:: 35967877

Documentos

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Métricas

Filiaciones

Seoane, R:: Univ Santiago Compostela, Ctr Invest Med Mol & Enfermedades Cron CIMUS, Inst Invest Sanitarias IDIS, Santiago De Compostela, Spain
Llamas-Gonzalez, YY:: Inst Conmemorat Gorgas Estudios Salud, Grp Biol Celular & Mol Arbovirus, Panama City, Panama

Univ Republica, Programa Doctorado Ciencias Biol, Montevideo, Uruguay
Vidal, S:: Univ Santiago Compostela, Ctr Invest Med Mol & Enfermedades Cron CIMUS, Inst Invest Sanitarias IDIS, Santiago De Compostela, Spain
El Motiam, A:: Univ Santiago Compostela, Ctr Invest Med Mol & Enfermedades Cron CIMUS, Inst Invest Sanitarias IDIS, Santiago De Compostela, Spain
Bouzaher, YH:: Univ Santiago Compostela, Ctr Invest Med Mol & Enfermedades Cron CIMUS, Inst Invest Sanitarias IDIS, Santiago De Compostela, Spain
Fonseca, D:: Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY USA
Farras, R:: Ctr Invest Principe Felipe, Oncogen Signalling Lab, Valencia, Spain
Garcia-Sastre, A:: Icahn Sch Med Mt Sinai, Dept Microbiol, New York, NY USA

Icahn Sch Med Mt Sinai, Global Hlth & Emerging Pathogens Inst, New York, NY USA

Icahn Sch Med Mt Sinai, Dept Med, Div Infect Dis, New York, NY USA

Icahn Sch Med Mt Sinai, Tisch Canc Inst, New York, NY USA

Icahn Sch Med Mt Sinai, Dept Pathol Mol & Cell Based Med, New York, NY USA
Gonzalez-Santamaria, J:: Inst Conmemorat Gorgas Estudios Salud, Grp Biol Celular & Mol Arbovirus, Panama City, Panama
Rivas, C:: Univ Santiago Compostela, Ctr Invest Med Mol & Enfermedades Cron CIMUS, Inst Invest Sanitarias IDIS, Santiago De Compostela, Spain

Ctr Nacl Biotecnol CNB CSIC, Cellular & Mol Biol, Madrid, Spain