Monitoring platelet function in marine mammals: Intracellular Ca 2+ mobilization as a biomarker of platelet activation.

Fecha de publicación: 01/01/2024 Fecha Ahead of Print: 01/10/2023

Autores de CIPF

Jose Enrique O'connor Blasco

Autor
Alicia Martinez Romero

Autor

Participantes ajenos a CIPF

Felipo-Benavent M
Álvaro-Álvarez T
Valls-Torres M
Rojo C
García-Párraga D
Rubio-Guerri C

Grupos de Investigación

Unidad Mixta CIPF-UV de Citómica

Abstract

Platelet functionality plays a crucial role in marine mammals. Alterations in platelet function can result from stress, pathologies, or exposure to xenobiotics, among others. The early detection of platelet function abnormalities is essential in these species to prevent advanced pathology and mitigate potential risks. Our main objective was to establish a range of physiological values of platelet function in bottlenose dolphins (Tursiops truncatus), beluga whales (Delphinapterus leucas), sea lions (Otaria flavescens) and walruses (Odobenus rosmarus). Intraplatelet Ca 2+ mobilization using adenosine diphosphate (ADP) as a platelet agonist was used as a platelet function biomarker, adapting the methodology previously described by us in dolphins (Felipo-Benavent et al., 2022) to the rest of the species. The assay was also adapted to a seal (Phoca vitulina). Numerical indicators of intraplatelet Ca 2+ mobilization kinetics were established, and statistical analyses were performed to compare the effects of species, sex, age, aquarium and species. Significant differences were observed between species, being the platelets of the sea lions the more reactive to the agonist. This work demonstrates the usefulness of this assay in the diagnosis or monitoring of animals with hemostatic diseases, showing two clinical cases in which intraplatelet calcium mobilization values were altered in marine mammals suffering haemorrhages. This assay may also serve as a means to monitor environmental changes and their potential impact on the health of marine mammal populations.

Datos de la publicación

ISSN/ISSNe:: 0145-305X, 1879-0089
Tipo:: Article
Páginas:: 105080-105080
DOI:: 10.1016/j.dci.2023.105080
PubMed:: 37844676

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Métricas

Filiaciones

Felipo-Benavent M:: Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Department of Biochemistry and Molecular Biology, University of Valencia, Av. de Blasco Ibáñez, 15, 46010, Valencia, Spain

Department of Biomedical Sciences, Faculty of Health Sciences, Universidad CEU Cardenal Herrera, Alfara del Patriarca, 46113, Valencia, Spain
O'Connor JE:: Laboratory of Cytomics, Joint Research Unit CIPF-UVEG, Department of Biochemistry and Molecular Biology, University of Valencia, Av. de Blasco Ibáñez, 15, 46010, Valencia, Spain
Álvaro-Álvarez T:: Veterinary Services, Oceanogràfic, Ciudad de las Artes y las Ciencias, C/Eduardo Primo Yúfera 1B, 46013, Valencia, Spain
Valls-Torres M:: Veterinary Services, Oceanogràfic, Ciudad de las Artes y las Ciencias, C/Eduardo Primo Yúfera 1B, 46013, Valencia, Spain
Rojo C:: Veterinary Services, Oceanogràfic, Ciudad de las Artes y las Ciencias, C/Eduardo Primo Yúfera 1B, 46013, Valencia, Spain
García-Párraga D:: Veterinary Services, Oceanogràfic, Ciudad de las Artes y las Ciencias, C/Eduardo Primo Yúfera 1B, 46013, Valencia, Spain

Research Department, Fundación Oceanogràfic de la Comunitat Valenciana, Ciudad de las Artes y las Ciencias, C/Eduardo Primo Yúfera 1B, 46013, Valencia, Spain
Martínez-Romero A:: Cytomics Technological Service, Príncipe Felipe Research Center, C/Eduardo Primo Yúfera 3, 46012, Valencia, Spain
Rubio-Guerri C:: Research Department, Fundación Oceanogràfic de la Comunitat Valenciana, Ciudad de las Artes y las Ciencias, C/Eduardo Primo Yúfera 1B, 46013, Valencia, Spain

Department of Pharmacy, Faculty of Health Sciences, Universidad CEU Cardenal Herrera, CEU Universities, Valencia, Spain

Keywords

ADP, Cetaceans, Flow cytometry, Fluo-4 AM, Haemostasia, Pinnipeds, Platelet

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