Exploring the neuroprotective potential of antimicrobial peptides from Dinoponera quadriceps venom against pentylenetetrazole-induced seizures in vivo.

Fecha de publicación: 01/01/2024 Fecha Ahead of Print: 27/11/2023

Autores de CIPF

Maria Mar Orzáez Calatayud

Autor

Participantes ajenos a CIPF

Paes LCF
Lima DB
Silva DMAD
Valentin JT
Aquino PEA
García-Jareño AB
Fonteles MMF
Martins AMC

Grupos de Investigación

Laboratorio de Terapias Dirigidas en Cáncer e Inflamación

Jefe/a de Grupo

Maria Mar Orzáez Calatayud

Abstract

Epilepsy affects around 50 million people worldwide and 30% of patients have difficulty controlling the disease. The search for substances that can fill the existing gaps in the treatment of epilepsy is of great importance. Arthropod venoms are promising sources for this purpose due to the presence of small peptides that modulate the activity of ion channels and neuron receptors. The aim of this study was to investigate dinoponeratoxins from the Dinoponera quadriceps ant venom (M-PONTX-Dq3a, M-PONTX-Dq3b and M-PONTX-Dq3c) as potential anticonvulsants. We evaluated them in a seizure model induced by pentylenetetrazole (PTZ) in male swiss mice. Interestingly, intraperitoneal treatment with each peptide increased the time until the first seizure and the percentage of survival, with M-PONTX-Dq3b showing the best results. M-PONTX-Dq3a was discarded due to the appearance of some signs of toxicity with the increase in malondialdehyde (MDA) levels in the striatum. Both, M-PONTX-Dq3b and M-PONTX-Dq3c decreased iNOS and TNF-a in the hippocampus. Notably, M-PONTX-Dq3c treatment decreased the levels of MDA and nitrite in the cortex and hippocampus. Our results indicate that, M-PONTX-Dq3b and M-PONTX-Dq3c have anticonvulsant activity and exhibit anti-inflammatory effects in epilepsy, offering new perspectives for biopharmaceutical development.

Datos de la publicación

ISSN/ISSNe:: 0041-0101, 1879-3150
Tipo:: Article
Páginas:: 107538-107538
DOI:: 10.1016/j.toxicon.2023.107538
PubMed:: 38030096

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Filiaciones

Paes LCF:: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal Do Ceará, Fortaleza, 60430372, Ceará, Brazil
Lima DB:: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal Do Ceará, Fortaleza, 60430372, Ceará, Brazil
Silva DMAD:: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal Do Ceará, Fortaleza, 60430372, Ceará, Brazil
Valentin JT:: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal Do Ceará, Fortaleza, 60430372, Ceará, Brazil
Aquino PEA:: Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, 60430-275, Ceará, Brazil
García-Jareño AB:: Targeted Therapies on Cancer and Inflammation Lab and Peptide Synthesis Platform, Centro de Investigación Príncipe Felipe, Valencia, 46012, Spain
Orzaéz M:: Targeted Therapies on Cancer and Inflammation Lab and Peptide Synthesis Platform, Centro de Investigación Príncipe Felipe, Valencia, 46012, Spain
Fonteles MMF:: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal Do Ceará, Fortaleza, 60430372, Ceará, Brazil

Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, 60430-275, Ceará, Brazil
Martins AMC:: Department of Clinical and Toxicological Analysis, Faculty of Pharmacy, Universidade Federal Do Ceará, Fortaleza, 60430372, Ceará, Brazil

Department of Physiology and Pharmacology, Faculty of Medicine, Federal University of Ceará, Fortaleza, 60430-275, Ceará, Brazil