tappAS: a comprehensive computational framework for the analysis of the functional impact of differential splicing

Autores de CIPF

• Lorena De La Fuente Lorente

  Autor

• Ángeles Arzalluz Luque

  Autor

• 

  Cristina Marti Ibáñez

  Autor

• Sonia Tarazona Campos

  Autor

• Pedro Salguero García

  Autor

• Alberto Manuel Lerma Aguilera

  Autor

• Ana Alastrue Agudo

  Autor

• Pablo Bonilla Villamil

  Autor

• 

  M Victoria Moreno Manzano

  Autor

• Ana Conesa Cegarra

  Autor

Participantes ajenos a CIPF

• Tardaguila, M
• del Risco, H
• Scott, R
• Newman, JRB
• Kosugi, S
• McIntyre, LM

Grupos de Investigación

• Laboratorio de Regeneración Tisular y Neuronal

  

  Jefe/a de Grupo

  M Victoria Moreno Manzano

• Unidad Asociada CIPF-CSIC Ruido Biológico y Plasticidad Celular

  

  Jefe/a de Grupo

  Francisco Jose Iborra Rodríguez

Abstract

Recent advances in long-read sequencing solve inaccuracies in alternative transcript identification of full-length transcripts in short-read RNA-Seq data, which encourages the development of methods for isoform-centered functional analysis. Here, we present tappAS, the first framework to enable a comprehensive Functional Iso-Transcriptomics (FIT) analysis, which is effective at revealing the functional impact of context-specific post-transcriptional regulation. tappAS uses isoform-resolved annotation of coding and non-coding functional domains, motifs, and sites, in combination with novel analysis methods to interrogate different aspects of the functional readout of transcript variants and isoform regulation. tappAS software and documentation are available at .